Speedily get hold of sections as thin or thick as your application calls for, without worrying about dropping or distorting worthwhile tissue samples.
Megatome is really a vibrating microtome intended to part a wide number of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With high blade vibrating frequency and minimized blade deflection, Megatome enables significant-throughput tissue sectioning with uniform area profile, as well as minimal tissue harm and data reduction.
Antibodies might consider months to diffuse by just a few millimeters of tissue, that has a steep labeling gradient from floor to Main.
Megatome is created for precision: the blade vibrates at a better frequency and larger amplitude assortment than other microtomes, and includes a special deflection Manage mechanism.
Clone the job or grab the most up-to-date release. Running the utility will range a little determined by the way you retrieve the project.
Our preformulated EasyIndex Remedy raises and homogenize the refractive index of delipidated tissue samples, rendering them entirely clear. This allows light penetration in to the sample and makes certain the acquisition of superior-resolution, in-target graphic info.
Megatome is often a novel microtome which allows for top-precision sectioning of a variety of tissue samples – from organoids, to arrays of animal organs, to intact human brain hemispheres – with minimum tissue injury and knowledge loss.
eFLASH is a fast tissue labeling method that permits for uniform entire-organ staining in 20 rounds of labeling.
Fully delipidate full mouse brains or megatomi.com comparably sized samples in only one working day with SmartBatch+, or in a single 7 days with our passive clearing package.
Docset development calls for at bare minimum two choices: the name of your docset and The situation on the Javadoc documents to include within the docset.
This tends to develop a docset named Sample in the current Listing. Docset generation could be personalized with optional arguments:
SHIELD avoids the variability of hydrogel embedding and the data reduction from PFA preservation, guarding specimens for various rounds of processing.
Leverage the Apparent+ tissue clearing strategy, along with eFLASH and patented stochastic electrotransport systems, to quickly crystal clear and label entire organs. Vital highlights and characteristics include:
Our novel Defend tissue preservation method varieties intramolecular bonds utilizing polyfunctional, adaptable epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.